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文献和实验Dual- and Triple-Color Fluorospot
in several publications and reagents/kits for various cytokine combinations and species are commercially available. The most common protocol for fluorospot includes the use of biotinylated detection antibodies for one cytokine and an FITC-labeled detection antibody
Immunohistochemistry: Fluorescence Protocol 2.0
temperature with 15 µL of a fluorochrome-labeled secondary antibody (either FITC-labeled anti-mouse IgG1 , IgG2A or IgG2B diluted 1:300) in Wash Buffer-Saponin supplemented with 5% human AB serum. Cells are ready for analysis by flow cytometry
Intracellular Cytokine Staining Protocol
stimulation period for induction of a given cytokine is variable and has to be determined. For example, the best time for detection of IL-6-producing cells by human LPS-activated monocytes is 6 hours, whereas IL-10 needs at least 24 hours stimulation
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