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文献和实验://www.sigmaaldrich.com/catalog/Lookup.do?N5=All&N3=mode+matchpartialmax&N4=PMA&D7=0&D10=PMA&N1=S_ID&ST=RS&N25=0&F=PR 到底是那个啊 中介代理说是1000, 我还没给钱 chu465292278 chenfengyiguo wrote: 对了 6:sigma主页网上说List Price RMB是1000元
Protocol for intracytoplasmic staining of cytokines for FACS analysis
. 2) Stimulate cells for 2 hours in vitro with 50 ng/ml PMA (Sigma, P8139) + 500 ng/ml ionomicin (Sigma I0634) in phenol red free RPMI 1640 medium + 10% fetal calf serum at 37 deg C. 3) Add Brefeldin A (Sigma, B7651) to 10mg/ml for a further 2 hr
Metabolic labeling Immunoprecipitation
Materials •Complete medium: DMEM supplmented w/ 10% FCS, NEAA, Na-pyruvate, and Gln •Expre35 S35 S Protein labeling mix (NEN,#NEG-072), 7.9mCi/ml • Transfection reagents: CaCl2 , x2HBS, DNA •Labeling Medium: RPMI1640(Met,Cys-free; Sigma R-7513
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