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上海玉博生物科技有限公司
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文献和实验, we aimed to highlight the basic knowledge on the use of three most commonly performed tracers, namely Evans blue dye, sodium fluorescein, and horseradish peroxidase. The experimental methodologies that we use in our laboratory for the detection
Fluorescein labeling of proteins
This method uses carboxyfluorescein succinimidyl ester (CFSE)rather than fluorescein isothiocyanate,resulting in more reliable labeling.Succinimidyl esters are excellent reagents for amine modification since the amide products formed are very stable
CFTR Regulation of Epithelial Sodium Channel
channel belonging to the ABC (A TP B inding C assette) superfamily of transporters. The members of this superfamily use ATP hydrolysis to fulfill their function as active transporters. So far, CFTR is the only member of this family to function as a cAMP
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