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文献和实验Isolation of Cotton Genomic DNA from Leaf Tissue
PVP 40,000 MW 0.1% DIECA, diethyldithiocarbamic acid (disodium salt) 0.2% beta-mercapto-ethanol or Na2 S2 O5 (add when used) Lysis Buffer 0.1 M Tris HCl (pH
. The cultures are then pelleted at ~2,000 RPM for 4 minutes and the pellet re-suspended in 500µl of cold lysis buffer on ice for 10 minutes and then centrifuged 5,000 RPM for 5 minutes at 4°C. The pellet is considered the nuclear fraction while the solution
. Accurate protocol for 100mM stocks preparation. add the necessary quantity (see table) of H2 O and Tris base 1M (it is possible to take the volume of salt as ~150µl); Mw V(H2O) V(Tris base 1M) V
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