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文献和实验Development of techniques for primary culture of C. elegans embryonic neurons
mixed-stage embryos were obtained from mixed-stage C. elegans populations by washing in M9 followed by treatment with 20% sodium hypochlorite solution in 0.5 M NaOH until all larvae and adults were dissolved. Embryos were washed several times
Detection of apoptotic process in situ using immunocytochemical and TUNEL assays
anti-DNA-POD (1: 5; 1: 10) overnight at 4°C. 7. Wash in PBS (3x5 min). 8. Demonstration of peroxidase activity in a Petri dish: dissolve a DAB tablet (10 mg) in 40 ml of 0.1M citrate acid-sodium citrate buffer (pH 5.5), add 5 ml of H2
Whole-mount in situ hybridization for the detection of RNA in C. elegans embryos
Whole-mount in situ hybridization for the detection of RNA in C. elegans embryos Geraldine Seydoux and Andrew Fire Adapted from Seydoux, G. and Fire, A. (1995). Whole-mount in situ hybridization for the detection of RNA in C. elegans
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