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文献和实验Load both sides of the hemacytometer with the cell suspension - covering both counting grids . Count the number of cells in 5 out of 9 squares
Fusion is an important procedure to produce monoclonal antibodies (MoAb).
). Just before the fusion wash 3 times in IMDM (serum free!), 1200 rpm, 5min, 37ѓC. Count cells. sacrifice the mouse and dissect the spleen. tease the spleen in ice-cold IMDM (serum free!); pass through the nylon filter; wash 3 times in IMDM (serum free
Fusion of Mouse, Rat, or Hamster Cell
Fusion of Mouse, Rat, or Hamster Cell Author: Nanci Donacki Source: Contributed by Nanci Donacki
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