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文献和实验cDNA sequencing based on PCR and random shotgun cloning
because once the sequence of a genomic DNA containing cosmid is obtained and putative exons are predicted, the corresponding cDNAs should be sequenced in a timely manner. However, the presently implemented directed cDNA sequencing strategies, i.e. primer walking (17
利用RACE [Rapid Amplification of cDNA ENDs,cDNA末端的快速扩增],可以很简单地得到mRNA的5'末端序列,然而5'RACE是一个很复杂的技术,它的成功依赖每一步反应的有效完成。cDNA第一链的合成是由一个反义的基因特异性引物(Gene-specific primer,GSP)来起始的,cDNA第一链纯化后,利用末端脱氧核苷酸转移酶(Terminal deoxynucleotidyl transferase,TdT)在cDNA的5'末端加上一个合成
Differential cDNA Screening Procedures(cDNA差异筛选)
The protocols listed refer to cDNA library construction and preliminary differential screening procedures. They are mainly adaptations of standard procedures as per Sambrook et al. (1989) where theoretical background information
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