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文献和实验A general protocol for staining cell for cytometry analysis
µl of 1X Binding Buffer and add to the cell pellet. Gently mix the cells. Add 2 µl PI and incubate for 15 min at RT. 5. Add 400 µl of 1X Binding Buffer to each tube. Analyze by flow cytometry as soon as possible (within 1 hr). Note
Balanced Salt Solutions a,b Ringer(c) Tyrode (de) Gey(f) Earle(g) Puck(h) Hanks(l)
The ribonuclease protection assay (RPA)
The ribonuclease protection assay (RPA) is a highly sensitive and specific method for the detection of mRNA species. The assay was made possible by the discovery and characterization of DNA-dependant RNA polymerases from the bacteriophages SP6, T
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