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文献和实验with a salt solution (300 mM NaCl) of high ionic strength. (C ) Ca2+ induces defects on a supported membrane containing 25% POPA. Defects appear upon addition of 0.5 mM CaCl2 and gradually increase in size with time. (D ) and (E ) Examples of diffusion
In Vitro Transcription Assay (Run-off Assay) using Permeabil
by centrifugation at 1,000 X g for 5 min at 4℃. 4. Aspire supernatant. Resuspend cell pellet in 10 ml of PBS. 5. Centrifuge at 1,000 X g for 5 min at 4℃. 6. Suspend cell pellet in Permeabilization Buffer to a cell concentration of 108 cells/ml. 7. Check
ELECTROPHORESIS OF DNA IN POLYACRYLAMIDE GELS
) 3 3.5 5 40% bis/acrylamide (ml) 3.75 4.4 6.2 Water (ml) 17.25 20.1 28.8 50% Glycerol (ml) 6 7 10 APS (mg) 34 40 57 TEMED (ul
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