LI-COR特约代理商|926-32210 IRDye® 8

Purification of mAb (IgG)

mlAdd 40 g ammonium sulfate for every 100 ml Sup. (slowly):800gStir O/N in cold room.Pour into plastic bottles. Spin at 4 ℃, 7,500 rpm for 20 min in JA-10 rotor.Prepare 4L 100 mM Tris/pH7.8 (400 ml of 1 M stock/4 L) or Binding buffer.Discard Sup. Resuspend

Purification of mAb (IgG)

buffer (pH9.0) ~ 200 ml. Filter protein mixture Add protein mixture or culture supernatant containing Ab (adjust pH to 7.8 with Binding buffer ; red color) to the Protein A column. Mouse antibodies of the IgG1 subclass

Technovit® 9100 Methyl Methacrylate

with buffer (see above)     DAKO EnVision polyvalent antibody (goat-anti-mouse/goat-anti-rabbit) coupled to alkaline phosphatase 30 min at room temperature Visualization Step