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Protocol for Silver Staining

.You should wear clean,disposable gloves and never touch the gel bands directly.The following is a protocol used at the proteomics resource center for Silver staining.If you are using Silver staining kit from Sigma,Invitrogen,or other venders,please follow

Silver Staining of PAGE Gels

-- 5 min 10 min 7. dH2O 400 ml -- -- 10 min 8. Silver reagent 200 ml 15 min 20 min 30 min 9. dH2O 400 ml -- 1 min 2 min 10. Developer 200 ml 30 sec. Develop until solution turns yellow or until a brown "smoky" precipitate appears. Then pour off developer

Silver Staining SDS PAGE Gels

  We currently use the SilverQuest Silver stain kit from Invitrogen. This method is faster than the method listed below. The rapid method works well for most applications. Click here for instructions to the SilverQuest kit Non-Kit based