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文献和实验Deglycosylation of Glycoproteins Using Endoglycosidases
even after denaturation in SDS. A typical test reaction on human transferrin is described in the protocol below. Materials: Transferrin Sodium Dodecyl sulfate Nonidet P-40 Gel electrophoresis apparatus
The Identification of Dengue Virus Using PCR
4) are circulating in tropical countries and cause dengue fever and dengue hemorrhagic fever, both of which are highly prevalent. Fig. 1. The effect of Nonidet P-40 treatment on dengue virus morphology. Dengue type-2 virus was purified by linear
SDS -PAGE and Western Blotting Techniques
can be solubilized by lysis buffers that contain the nonionic detergent Nonidet P-40 (NP-40) and either no salt at all or relatively high concentrations of salt (e.g., 0.5 M NaCl). However, the efficiency of extraction is often greatly affected by pH of the buffer
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