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上海玉博生物科技有限公司
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文献和实验with about 2-nm chromium undercoat. The silver films were protected with 5 nm layer of silica. Poly-l-lysine solution was freshly prepared solution: 8 ml water 1.0 ml Na–phosphate buffer, 50 mM, pH 7.4, 1.0 ml poly-l-lysine solution (0.1%, Sigma). 实验
-based Analysis)Proteinase K (Sigma)Proteinase K buffer (0.05 M tris-HCL, 0.001 M EDTA, 1% Tween 20, 0.1 mg/ml proteinase K, pH 8.0)Ampli Taq Gold Buffer (Perkin Elmer)dNTP mixture (Perkin Elmer)PrimersDEPC-treated H2OAmpli Taq Gold Polymerase (Perkin
Primary Amplification of Genomic DNA using DOP - PCR
Reagents Agarose, Ultrapure Gibco, BRL, Cat. no. 15510-027 10X Buffer, and Perkin Elmer (Part no. N808-0010) Template DNA Use 50-100 ng for each reaction Ethidium Bromide Research Genetics, Cat. no. 750007 Loading buffer, 5X
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