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文献和实验Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
(95K): [in this window] [in a new window] Figure 2. Distribution of the Polycomb protein at section 43-49 of the second chromosome visualized with enzyme-conjugated (HRP) secondary antibodies. (Top ) Chromosome
Prediction of Protein‐Protein Interaction Networks
, S.F., Madden, T.L., Schaffer, A.A., Zhang, J., Zhang, Z., Miller, W., and Lipman, D.J. 1997. Gapped BLAST and PSI‐BLAST: A new generation of protein database search programs. Nucleic Acids Res. 25:3389‐3402.
Purification of Kar3 Motor Domain Protein
NaCl (6 x 1 mL fractions). The bulk of the protein elutes with 200 mM NaCl in the 2nd to 4th fractions (fr 200-2 to 200-4) and can be ~90-95% pure. Add 5 microliters of 200 mM PMSF to each peak fraction. 9. Pool peak
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