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文献和实验Metabolic Labeling of Cells with 35S
7) Centrifuge briefly. 8) Add 100l of a 75:25 protein A:50mM Tris mixture. 9) Incubate at least 1 hour using the mixer located at cold room. 10) Centrifuge briefly. 11) Remove the supernatant using a 5 ml syringe with a 26 1/2 G needle. 12) Add 1 mL
Typical modification sites: RGG box or RXR sequence motifs R-arginine, G-glycine, X-any aminoacid. Enzymes catalysing protein arginine methylation: PRMT1 from rat and the human homologue HRMT1L2, human PRMT2 (HRMT1L1), rat and human PRMT
5. (take residue) extract at +4 oC with H2O (2x) and filter 6. (take residue) extract at +20-25 oC with acetone (5x) and filter 7. (use muscle acetone powder) extract with buffer G at 0 oC; 20 ml/7 gram acetone powder; centrifuge at 35,000 x g for 30 min
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