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文献和实验Targeted Sequencing Using Affymetrix CustomSeq Arrays
solutions (Roche cat. no. 1969064; store at −20°C), and combine equal volumes of the four 10 mM dNTPs 10 µM synthesized oligonucleotide primer mix: dilute 100 µM stocks solutions (Integrated
蛋白复合体直接酶消化法Direct Enzymatic Digestion of Protein Complexes
8.0) Enzymes Elastase Grade II Endoproteinase Lys-C Purified complex of interest Subtilisin Trypsin, sequencing grade, modified Special Equipment
Cycle Sequencing Protocol Using 33P-Radiolabeled Terminators
tends to produce high background and ghost bands.) 1.Prepare the 4 termination mixes by combining 2.0ul of dGTP-termination-master-mix and 0.5ul of labeled ddNTP for each sequence. 2.For each set of sequencing reactions, label four PCR grade microfuge
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