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文献和实验ELISPOT (Enzyme-linked ImmunoSPOT)
-specific responses, applicable to a wide range of areas of immunology research, including cancer, transplantation, infectious disease, and vaccine development. Human IL-17
/RecT from the Rac phage or Redα/Redβ from the λ phage, to assist in the cloning or subcloning of fragments of DNA into vectors without the need of restriction enzyme sites or ligases (7,8). A limitation of the original homologous recombination technique
Translocation Assays of Protein Kinase C Activation
, and γ), the Ca2+ -independent (nPKCδ,ɛ,η,θ, and μ), and phorbol ester-insensitive (aPKCζ and ι, the human counterpart of mouse PKCλ) subfamilies. Much research on this growing family of protein kinases has concentrated on the possibility
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