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文献和实验800/1200 L0007 Mouse anti-Human CD3 mAb, Biotin 100ug/500ug 800/2500 L0008 Mouse anti-Human CD4 mAb,FITC 50T/100T 800/1200 L
Intracellular Cytokine Staining Protocol
TimeRestimulationIntracellular BlockAntibodyIL-1aNEW!mouse PECmINFg (100ng/ml)(2h4)/LPS (100ng/ml)(22hr)2hr/22hr-MonensinALF-161IL-1bNEW!mouse PECmINFg (100ng/ml)(2h4)/LPS (100ng/ml)(22hr)2hr/22hr-MonensinB122IL-2mouse spleenConA (3ug/ml) (2d)/IL-2 (20ng/ml)+IL-4 (20ng/ml) (3d
Intracellular Immunofluorescent Staining for Flow Cytometry
11): Left: Human peripheral blood cells were stimulated for 2 days with IL-2. The cells were surface stained with FITC CD56 (MEM188) (Cat. No. 11-0569 ) and stained intracellularly with PE GB11. Quadrants demarcate boundary for isotype controls.
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