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上海玉博生物科技有限公司
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文献和实验Measurement of HAMA and Anti-Idiotypic Antibodies
). Even in the absence of such immunization some patients may possess HAMA activity because of polyclonal IgM rheumatoid factors that crossreact with Fc epitopes on both human and mouse IgG (1 ).
Dual- and Triple-Color Fluorospot
labeled with green fluorophore emitting light at the same wavelength as FITC, streptavidin (SA) labeled with Cy3, and if three cytokines are analyzed, a mouse anti-tag mAb labeled with Cy5 (Mabtech). 5. Mouse mAb anti-human CD28 at 0.1 mg/ml
Flow Cytometric Analysis Of Bcl Family members
against Bcl-xL (Santacruz Biotechnology, USA) and rabbit polyclonal against Bax (Santacruz Biotechnology, USA). The secondary AB against Bcl2 was goat anti mouse Ig FITC (Immunotech, USA) and against Bcl-xL and Bax goat anti rabbit FITC (ICN-Cappel, USA). FITC
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