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文献和实验Fragment, exo-) in the presence of labeled deoxyribonucleoside triphosphates. Using Klenow Fragment, exo- the reaction time can be prolonged without the fear of labeled probe degradation.The HexaLabel™ DNA Labeling Kit offered by Fermentas is an efficient
Construction of Gene-Targeting Vectors by Recombineering
plates using LB agar and antibiotic concentrations specified above. Prepare LB-Bsd plates using Fast-Media Kit. Prepare LB-Puro-Kan plates using Fast-Media Kit and add Kan to final concentration of 30 µg/mL. SOC medium (Invitrogen) TOP10
Long PCR Reagents and Guidelines长距离PCR反应溶液和反应条件
polymerase is the main polymerase in the reaction, and a proofreading polymerase (3' to 5' exo) is present at a lower concentration. Following the results of Cheng et al. PCR_protocol.html#Cheng">(1) we have had success using Tth (ABI/Perkin-Elmer
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