thermo特约代理商|22589 Sulfo-SANPAH

蛋白偶联到羧基化微球的方法

by microcentrifugation at ≥ 8000 x g for 1-2 minutes.25.Repeat steps 23. and 24. This is a total of two washes with 1 mL PBS-TBN.26.Remove the supernatant and resuspend the coupled and washed microspheres in 250-1000 μL of PBS-TBN. 27.Count the microsphere

蛋白偶联到磁性MagPlex™微球的方法

the stock uncoupled microsphere suspension according to the instructions described in the Product Information Sheet provided with your microspheres. 2.Transfer 5.0 x 106 of the stock microspheres to a USA Scientific microcentrifuge tube.

Studies of the Ubiquitin Proteasome System

or other proteasome inhibitor (e.g., lactacystin; Calbiochem or Biomol) Phosphate‐buffered saline (PBS; see recipe ) Nondenaturing Triton X‐100 lysis buffer (see recipe ) containing 1×