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文献和实验Dynamic Monitoring of Cellular Remodeling Induced by the Transforming Growth Factor-β1
Signaling and Sigma-Aldrich). Anti-β-actin (A5441) was from Sigma-Aldrich; horseradish peroxidase-conjugated anti-mouse IgG (#NA931) and anti-rabbit IgG (#NA934) were from GE Healthcare. Detection of antibody reactivity was performed using Immobilon Western
v468 Chapter 6 细胞内结肠腺瘤样息肉蛋白核定位以及细胞质检测
: anti-mouse or anti-rabbit conjugated tobiotin from Dako Corp. (Glostrup, Denmark) diluted 1:500. 8. Third antibody: Texas Red conjugated to avidin from VectorLaboratories (Burlingame, CA) diluted 1:500. 9. Nuclear stain: 0.05 μg/mL of Hoechst
Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum
/) 96‐well plate (8 well removable strips) coated with polyclonal rabbit anti‐PINP antibody (from Competitive ELISA kit; IDS Ltd, http://www.idsplc.com/) Precision
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