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文献和实验Fastfilter Plasmid Maxi Kit Spin Protocol
实验步骤 Growth of bacterial culture: 1. Culture volume: Inoculate 200 ml LB/ampicillin (50 ug/ml) medium placed in a 1-4 liter culture flask with E.coli carrying desired plasmid and grow at 37°C with agitation for 12-16
Fastfilter Plasmid Midi Kit Spin Protocol
实验步骤 Growth of bacterial culture: 1. Culture volume: Inoculate 30-50 ml LB/ampicillin (50 ug/ml) medium placed in a 200-400 milliliter culture flask with E.coli carrying desired plasmid and grow at 37°C
Reprogramming Fibroblasts with the CytoTune-iPS Reprogramming Kit
实验步骤 1. Preparing MEF dishes 1) Gelatin coating culture vessels a. 1. Cover the whole surface of each new culture vessel with Attachment Factor (AF) solution and incubate the vessels for 30 minutes at 37°C
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