santa特约代理商|sc-108062 20ml Pla

Procedure for Transfection of Mammalian Cells

in a CO2 incubator until the cells are 70-80% confluent. This will usually take 18-24 h. Prepare the following solutions in 12 x 75 mm sterile tubes: Solution A: For each transfection, dilute 2 μg DNA (plasmid) in 375 μl serum-free IMDM

Plasmid

This protocol is for Mini (up to 20 µg) preparations of high-copy plasmid DNA from cultures of E. coli . Important notes before starting New users are strongly recommended to read Appendix A (page 21) at the end

【原创】lentivirus calcium phosphate transfection protocl

calcium phosphate transfection protocl： 1.  Split 293FT cells about 30% confluence, overnight. 2.  Thaw 2*HBS room temperature (not in 37 C). 3.  For 10cm dish: 1.5ml tube, 4ug packaging plasmid A, 4ug packaging plasmid B, 4ug expression