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文献和实验Procedure for Transfection of Mammalian Cells
in a CO2 incubator until the cells are 70-80% confluent. This will usually take 18-24 h. Prepare the following solutions in 12 x 75 mm sterile tubes: Solution A: For each transfection, dilute 2 μg DNA (plasmid) in 375 μl serum-free IMDM
This protocol is for Mini (up to 20 µg) preparations of high-copy plasmid DNA from cultures of E. coli . Important notes before starting New users are strongly recommended to read Appendix A (page 21) at the end
【原创】lentivirus calcium phosphate transfection protocl
calcium phosphate transfection protocl： 1. Split 293FT cells about 30% confluence, overnight. 2. Thaw 2*HBS room temperature (not in 37 C). 3. For 10cm dish: 1.5ml tube, 4ug packaging plasmid A, 4ug packaging plasmid B, 4ug expression
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