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Molecular Methods: Chip Assay and Quantitative Real-Time PCR: In Detecting Hepatotoxic Cyanobacteria

Cyanobacterial mass occurrences are widespread and often contain hepatotoxic, i.e. microcystin- and nodularin-producing, species. Nowadays, detection of microcystin (mcy ) and nodularin synthetase (nda ) genes is widely used

液相色谱-电喷雾串联四极杆质谱法检测水体中微囊藻毒素

，为定量、定性离子的选择提供了理论依据。但未涉及到如何准确定量。MoucunYuan等人利用SELDI-TOF质谱对MCYST-LR、YR、RR和nodularin进行分析，利用Ciphergenchips可使绝对检测量（LOD）达10pg左右的。K.P.Bateman等应用毛细管电泳分离技术与MS/MS串联对主要的生物和肽类毒素进行了分离，以此作为对LC和ESMS分析方法的补充。有关应用液相色谱与三重四极杆液质联用技术，应用MRM检测方式对低含量MCYST样品进行准确定量方法学研究至今还未见详细

Strategies to Optimize Protein Expression in E. coli

phosphatase‐1 bound to nodularin‐R and tautomycin: A novel scaffold for structure‐based drug design of serine/threonine phosphatase inhibitors. J. Mol. Biol. 385:11‐21.    Kikuchi, Y