epicentre特约代理商|MP5120 MAXPLAX™

Use of Cosmid Libraries in Plant Transformations

to be packaged in vitro using commercially available highly efficient packaging extracts, just as lambda libraries can. The high efficiency of in vitro lambda packaging allows a cosmid library with large inserts to be more easily constructed than a simple plasmid

Differential cDNA Screening Procedures

0.5 ul Incubate at 10-150C overnight continue at RT the next day. 9. Packaging follows next day. * Prepping the Lambda vector before cloning. 1. We prepare 25 ug of lambda vector. The Lambda DNA is (25ug/100 ul of O.1 X TE) heated to 68 C

基因组DNA文库构建的基本流程

细胞 如果使用Epicentre试剂盒构建黏粒文库，需要用Epicentre MaxPlax Lambda Packaging Extracts 来包装上述的连接反应产物，测定包装好的黏粒克隆的滴度，然后转染Epicentre EPI300-T1?Plating Strain。挑出重组子，鉴定插入片段的大小。如果文库的大小和质量都令人满意的话, 就可以铺板进行文库筛选，或者扩增、保存文库。 如果使用Epicentre试剂盒构建BAC文库，应选择电转法，可以选择