thermo特约代理商|77600 Imject™ mcKL

蛋白偶联到磁性MagPlex™微球的方法

  Sample Protopcol  for  Two-Step Carbodiimide  Coupling   of  Protein to MagPlex™ Magnetic  Carboxylated Microspheres Microspheres should be protected from prolonged exposure to light throughout this procedure. 1.Resuspend

ELISPOT标准操作程序（protocol）

。（1）本次洗涤目的在于：除去预湿用的乙醇。因此应该尽量洗涤干净，减少残留。（2）包被：按说明书操作。50μL/孔加入用1×PBS稀释好的包被抗体，4°C包被过夜。5、 封闭：（次日）倾倒包被液，用无菌1×PBS洗涤3次。最后一次，在灭菌的吸水纸上扣干。200μL/孔加入稀释好的封闭液，37°C封闭1h。6、倾倒封闭液，无须洗涤，直接加入检测细胞进行ELISPOT检测。二、Magi™ Coating Buffer润湿包被程序该方案中，用Magi™ Coating Buffer润湿PVDF孔后，直接加入

SPRi在核酸适配体开发中的应用

DNA was immobilized on the planar gold SPRi-Biochip™ sensor through thiol-Au chemisorption.The aptamer solutions were prepared at 10 μM in 20 mM PBS pH 7.4.Four rep-licate spots were printed simultaneously for each aptamer using the SPRi-CFM (Figure