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文献和实验V294.PART II,Chapter 4 细胞迁移实验之Dunn趋化迁移培养皿法及时序显微镜分析法
consists of RPMI1640 withL-glutamine, 1% essential amino acids, 1% sodium pyruvate, 1% penicillin–streptomyosin, 10% heat-inactivated fetal calf serum, and 0.5% β-mercaptoethanolsolution. The β-mercaptoethanol solution (50 mM) consists of 34 μL of14.3 M β
Constructing a Low-budget Laser Axotomy System to Study Axon Regeneration in C. elegans
to control focus 1) This system uses dual Galilean beam expanders to expand the laser beam to fill the back aperture of the objective (10 mm). Mount the 4 lenses on to carriers and attach them to the rail (L1f1 L2f2 and L3f1' L4f2'). Adjust
,NJ), loaded with Cu 2+ . 3. Binding Buffer: 0.5 M NaCl, 20 mM Tris-HCl, pH 7.5, and1% (w/v) CHAPS. 4. Elution Buffer: Binding Buffer with 100 mM imidazole, pH 7.5. 5. Syringe (10 mL) with 0.2-μm pore size filter. 2.3.3. Step 3: Gel
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