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文献和实验Measuring the Radioactivity of 2-D Protein Extracts
The in vivo radioactive labeling of proteins is used to enhance the sensitivity of detection and to quantitate the abundance individual proteins. To compare the quantity and identity of resolved proteins using 2-D gels, identical amounts
Nonreducing 2-D Polyacrylamide Gel Electrophoresis
that some monoclonal antibodies (MAbs) recognize only conformational epitopes, which are dependent on the native spatial conformation of the protein, as distinct from sequential determinants, which are dependent on the linear amino acid sequence of a peptide
Solubilization of Proteins in 2-D Electrophoresis: An Outline
The solubilization process for 2-D electrophoresis has to achieve four parallel goals: 1. Breaking macromolecular interactions in order to yield separate polypeptide chains: This includes
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