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文献和实验Expression Profiling Using Affymetrix GeneChip Probe Arrays
Large-scale microarray expression profiling studies have helped us to understand basic biological processes and to classify and predict the prognosis of cancers; they have also accelerated the identification of new drug targets. Affymetrix
In Situ Hybridization Histochemistry Using Alkaline Phosphatase-Labeled Oligodeoxynucleotide Probe
expression at individual cellular lev- els. For the detection of a particular target mRNA, a specific probe that has complementary sequence to the target mRNA is used. This probe has to include a reporter molecules in order to visualize the position
Cell-Based Assays to Probe the ERK MAP Kinase Pathway in Endothelial Cells
To understand signaling pathways in mammalian cells, cell-based assays are relatively new and extremely powerful tools. We have developed a battery of phenotypic assays to study signaling; two of them are described in detail in this chapter
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