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文献和实验The Cell Environment (including types of culture medium)
GMEM (Prod. No. G5154) Glasgows modified MEM was defined for BHK-21 cellsComplex media RPMI 1640 (Prod. No. R0883) Originally derived for human leukaemic cells. It supports a wide range of mammalian cells including hybridomas
, M. and Alonzo, N. 1983. Analysis for methylated amino acids in proteins. Methods Enzymol. 91:8‐13. Feeney, R.E. 1987. Chemical modification of proteins: Comments
Synthesis and Application of Highly Reactive Amino Linkers for Functional Oligonucleotides
2 ) 1 M sodium phosphate buffer (pH 8) Sterilized H 2 O Fluorescein isothiocyanate (FITC; Dojindo
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