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文献和实验Keratin 19 as a Stem Cell Marker In Vivo and In Vitro
such as keratin 19 also permits their study in human tissues. In this chapter, protocols to study skin stem cells using their property of slow cycling and their expression of keratin 19 will be described in detail. The methods include the double labeling
Identification of Epithelial Stem Cells In Vivo and In Vitro Using Keratin 19 and BrdU
the localization of skin stem cells in the bulge region of the pilosebaceous units and in the bottom of rete ridges in glabrous skin. The development of markers such as keratin 19 also permits their study in human tissues. In this chapter, protocols to identify
7.4）TBS洗10min.（10）兔抗SPAIgGl:400稀释，37℃ 30min.（11）0.05mol/L（pH7.4）TBS洗10min.（12）滴加PAGlonm 1:60稀释，室温1h,或37℃ 30min.（13）0.05mol/L（pH7.4）TBS洗10min.（14）1%戊二醛洗10min.（15）双蒸水洗5min.（16）1%明胶洗5min.（17）银避光显影8~15min. （18）双蒸水洗15min. （19）固定：用显影定影液（1:4或1:10）固定5min,也可以用15
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