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Targeted Exon Sequencing by In‐Solution Hybrid Selection

a protocol for the targeted enrichment of exons from randomly sheared genomic DNA libraries using an in?solution hybrid selection approach for sequencing on an Illumina Genome Analyzer II. The steps for designing and ordering a hybrid selection oligo pool

Identification of a Mutant Kinase/ATP Analog Pair

. Ponceau S solution 0.5% (w/v) in 1% acetic acid 2x Laemmli sample buffer 100 mM Tris (pH 6.8) 2% SDS 20% glycerol 4% β-mercaptoethanol (added fresh)

v468 chapter 5 糖原合成酶激酶-3 的抑制

serum (FBS;HyClone, Ogden, UT) and L -glutamine (Gibco/BRL). 2. Phosphate-buffered saline (PBS) containing 0.5 M ethylenediaminetetraacetic acid (EDTA). 3. Solution of trypsin (0.25%) containing 1 mM EDTA (Gibco/BRL). 4. Western lysis buffer