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上海玉博生物科技有限公司
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文献和实验Polyclonal and Monoclonal Antibodies
later again (2 + 2 week cycle). Monoclonal Antibody If titre is positive do one of the following 2 weeks later: I - Boost tail vein with 20 ug- 50 ug of Antigen in PBS and proceed with fusion on day 4. OR II - Boost
. Goding. 3. Monoclonal antibody technology; Ailsa M. Campbell. 上一篇:monoclonal antibody production fusion 下一篇:Polyclonal and Monoclonal Antibodies
Expanding Hybridoma(杂交瘤细胞) Clones
that the subclone is monoclonal, checking the isotype is possible . If not convinced that the hybrydoma is monoclonal, reclone a second time. Cloning of Hybridoma Make an accurate cell count of cells in log phase (3 E5 - 1E6 cells / ml).
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