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上海玉博生物科技有限公司
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文献和实验of choice (15µg, Lipofectamine 2000™ Invitrogen). The following day the flag-tagged protein expressing plasmid transfected cultures are transfected again with 100nM EF52 biotin labeled siRNA (antisense or sense alone) using Lipofectamine 2000™ or stock 3.4µM
Preparation and purification of biotin-labeled probe for ISH
Preparation and purification of biotin-labeled probe for ISH I. Biotin-labeling of plasmid or genomic DNA. Suggested chemicals: Nick Translation Kit, Enzo Diagnostics Cat. No. 42803. Prepare the following
® TACS Agarose Column, 1 ml(Cat.no 6 - 6310 - 001) 10x Buffer CI, 85 ml (Cat.no 6 - 6320 - 085) Anti-hu CD235a Biotin mAb Anti-hu CD14 Biotin mAb Anti-hu CD3 Biotin mAb
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