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上海玉博生物科技有限公司
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文献和实验Modification of the 5′ Terminus of Oligonucleotides for Attachment of Reporter and Conjugate Groups
Abstract Table of Contents Figures Literature Cited Abstract Reporter and conjugate groups
IMMUNOSCREENING OF WITH ANTIBODY PROBES
. Incubate these membrane sections with secondary antiserum for 1 hour at room temperature (For detecting human antibodies, we use Sigma #A-3150: goat anti-human IgG alkaline phosphatase conjugate, diluted 1:1000) Wash membranes in TBST for 5 minutes
secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
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