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文献和实验Purification of GST-fusion protein
Purification of GST-fusion protein This protocol uses Pharmacia glutathione sepharose matrix. Other companies also sells GST beads, e.g. Sigma. 1) Spin down cells and resuspend in ice-cold PBS (40 ml per 1 L of culture). Lyse
Molecular Assembly-Assisted Biocatalytic Reactions in Ionic Liquids
of ILs by the surfactant layer. In this chapter, a simple and effective method for the development of aqueous microemulsion droplets in a hydrophobic IL comprising an anionic surfactant sodium bis(2-ethyl-1-hexyl) sulfosuccinate (AOT) is presented
Balanced Salt Solution, 10ml bottle, store at 4 °C. Collagenase: SIGMA cat# C-9263, 100mg bottle. EDTA: SIGMA cat# ED2SS. Ethylenediaminetetraacetic acid, disodium salt, dihydrate, 100g bottle. Ethidium Bromide: SIGMA cat# E-8751. 2,7-diamino-10
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