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文献和实验Isolate It All：siRNA • miRNA • Total RNA • Native Protein
Figure 2. Analysis of Total RNA Isolated with the mir Vana ™ PARIS ™ Kit. Total RNA was isolated from 1 x 106 HeLa cells with the indicated kit as per protocol. (A) 1 µg of total RNA was resolved on a 15% denaturing acrylamide gel and stained
and measure protein concentration (BioRad assay) use 1 - 5 mg protein per IP immunoprecipitate for 2 h to ON, 4 °C wash immunoprecipitations pelleting the beads each time: 2 x 1 ml CHIP lysis buffer 2 x 1 ml CHIP
of choice (15µg, Lipofectamine 2000™ Invitrogen). The following day the flag-tagged protein expressing plasmid transfected cultures are transfected again with 100nM EF52 biotin labeled siRNA (antisense or sense alone) using Lipofectamine 2000™ or stock 3.4µM
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