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文献和实验Methods for the Detection of D-Amino-Acid Oxidase
according to the method of Laemmli (6 ) on a polyacrylamide-gradient (8-16%) slab gel (Tefco, Tokyo) with the electrophoresis buffer (25 mM Tris, 192 mM glycine, and 0.1% SDS) at 20 mA for about 1.5 hr. Hog kidney D-amino-acid oxidase (10 ng, Sigma) and prestained protein
Methods for the Detection of D-Amino-Acid Oxidase
were mixed with an equal volume of the sample buffer (63 mM Tris-HCl, pH 6.8, 2% SDS, 5% 2-mercaptoethanol, 10% glycerol and 0.002% bromophenol blue) and were heated in a boiling water for 3 min. The samples (5~50 μg protein per lane) were electrophoresed
BenchMark™ MagicMark™ XP new IEF mARKER 3-10
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