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p-NPP Single Reagent, Yellow,

Alkaline Phosphatase Substrate (soluble); 500 mL
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  • 询价
  • ES009-500ML
  • 2026年01月25日
  • elisa
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      elisa

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    • 库存

      大量

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    • 规格

      500 mL

    Description:
    p-NPP Single Reagent, Yellow, Alkaline Phosphatase Substrate (soluble)
    Trade Name:
    Chemicon (Millipore)
    Key Applications:
    ELISA
    Application Notes:
    p-Nitrophenyl phosphate (p-NPP) is the customary product utilized for the assay of alkaline phosphatase. It is usually supplied in tablet or powder form and is dissolved in an appropriate buffer immediately prior to assay. Liquid p-NPP concentrates are also available, but must also be diluted in buffer. CHEMICON, Inc. now provides p-NPP stabilized in diethanolamine (DEA) buffer and available for instant use. The DEA buffer contains Mg++ and Zn++ ions providing optimum activation of alkaline phosphatase labeled probes.

    This is an excellent substrate for phosphatase based ELISA assays.

    It produces a soluble end product which is yellow in color and reads at 405 nm to 410 nm. Both kinetic and end point determinations can be performed.
    Usage Statement:
    Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    Color Code:
    Yellow
    Presentation:
    p-NPP, a single component substrate in proprietary stabilizer.
    Storage Conditions:
    Maintain at 2-8°C for up to one year.
    Reagent Type:
    • ELISA Reagents
    • Enzyme Substrates
    Protein or Enzyme Type:
    Enzyme Substrates

    Product Family Information

    Luminata™ ELISA HRP Substrates
    Sensitivity and dynamic detection range for even your most demanding assays
    Sensitivity and dynamic detection range for even your most demanding assays.

    » More…


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    详细描述见链接:http://www.millipore.com/catalogue/item/ES009-500ML

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    图标文献和实验
    相关实验
    • DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE

      in the developing striatum. Neuron 9 :15. Henthorn, P., P. Zervos, M. Raducha, H. Harris, and T. Kadesch. 1988. Expression of a human placental alkaline phosphatase gene in transfected cells: Use as a reporter for studies of gene expression. Proc. Natl

    • (共享)ELISA原理与实验方法

      , incubate for 30 min at 37°C. ( 5g powdered milk in 95 ml PBS)5 Rinse with PBS 3x.6 Add 50 µl of antibody, incubate 30 min at 37°C.7 Rinse with PBS 3x.8 Add 50 µl of goat anti-mouse IgG conjugate with alkaline phosphatase.(1 µl of conjugate in 4 ml of PBS

    • Preparation of BAC DNA with by Alkaline Prep Method

      of isolation 1. Grow 250 ml of 2X YT culture at 37 ℃for 16 hours (> 250 rpm) with the appropriate antibiotic. Cultures can be started by 1) picking a single colony or 2) by splitting a subculture into 250 ml of culture (1 L Flasks). Colonies should be picked

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