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- 应用范围:
elisa
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大量
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500 mL
This is an excellent substrate for phosphatase based ELISA assays.
It produces a soluble end product which is yellow in color and reads at 405 nm to 410 nm. Both kinetic and end point determinations can be performed.
- ELISA Reagents
- Enzyme Substrates
Product Family Information
Luminata™ ELISA HRP SubstratesSensitivity and dynamic detection range for even your most demanding assaysSensitivity and dynamic detection range for even your most demanding assays. |
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详细描述见链接:http://www.millipore.com/catalogue/item/ES009-500ML
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文献和实验DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
in the developing striatum. Neuron 9 :15. Henthorn, P., P. Zervos, M. Raducha, H. Harris, and T. Kadesch. 1988. Expression of a human placental alkaline phosphatase gene in transfected cells: Use as a reporter for studies of gene expression. Proc. Natl
, incubate for 30 min at 37°C. ( 5g powdered milk in 95 ml PBS)5 Rinse with PBS 3x.6 Add 50 µl of antibody, incubate 30 min at 37°C.7 Rinse with PBS 3x.8 Add 50 µl of goat anti-mouse IgG conjugate with alkaline phosphatase.(1 µl of conjugate in 4 ml of PBS
Preparation of BAC DNA with by Alkaline Prep Method
of isolation 1. Grow 250 ml of 2X YT culture at 37 ℃for 16 hours (> 250 rpm) with the appropriate antibiotic. Cultures can be started by 1) picking a single colony or 2) by splitting a subculture into 250 ml of culture (1 L Flasks). Colonies should be picked
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