In Vitro Angiogenesis Assay Ki

In Vitro Angiogenesis Assay Kit

Chemicon (Millipore)

Angiogenesis is the process of generating new capillary blood vessels. It is a fundamental component of a number of normal (reproduction and wound healing) and pathological processes (diabetic retinopathy, rheumatoid arthritis, tumor growth and metastasis).

The CHEMICON In Vitro Angiogenesis Assay Kit provides a convenient system for evaluation of tube formation by endothelial cells in a convenient 96-well format. When cultured on ECMatrix™, a solid gel of basement proteins prepared from the Engelbreth Holm-Swarm (EHS) mouse tumor, these endothelial cells rapidly align and form hollow tube-like structures. Tube formation is a multi-step process involving cell adhesion, migration, differentiation and growth.

ECMatrix™ consists of laminin, collagen type IV, heparan sulfate proteoglycans, entactin and nidogen. It also contains various growth factors (TGF-beta, FGF) and proteolytic enzymes (plasminogen, tPA, MMPs) that occur normally in EHS tumors. It is optimized for maximal tube-formation.

Application:

The CHEMICON In Vitro Angiogenesis Assay Kit represents a simple model of angiogenesis in which the induction or inhibition of tube formation by exogenous signals can be easily monitored. For assaying inhibitors or stimulators of tube formation, simply premix the endothelial cell suspension with different concentrations of the inhibitor or stimulator to be tested, before adding the cells to the top of the ECMatrixTM. The assay can be used to monitor the extent of tube assembly in various endothelial cells, e.g. human umbilical vein cells (HUVEC) or bovine capillary endothelial (BCE) cells.

The CHEMICON In Vitro Angiogenesis Assay Kit is intended for research use only; not for diagnostic or therapeutic applications.

Activity Assay

Vertebrates

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Cell Based Assays

• ECMatrixTM Gel Solution: (Part No. 90060) Five 1 mL bottles.
• ECMatrixTM Diluent Buffer, 10X: (Part No. 90061) One 1 mL bottle.

Store kit materials at -20°C for up to 6 months. Once ECMatrix™ has been thawed, store at 2-8°C for one week. Do not refreeze.

1. 96-well Tissue Culture plate

2. Microcentrifuge Tubes, sterile

3. 37°C Tissue Culture Incubator

4. Inverted Light Microscope

5. HUVEC cells or other experimental cell line (any species)