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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
2-8°C or -15 to -30°C
- 保质期:
N/A
- 英文名:
Ready-to-use Sample and antibody dilution buffer
- 库存:
部分现货库存
- 供应商:
青木生物技术(武汉)有限公司
- 规格:
50 ml/125 ml/500 ml
| 规格: | 50 ml | 产品价格: | ¥805.0 |
|---|---|---|---|
| 规格: | 125 ml | 产品价格: | ¥1817.0 |
| 规格: | 500 ml | 产品价格: | ¥6348.0 |
Sample Buffer is designed for trouble free assays without interference. If you have background or false-positives – for example derived from cross-reactivities or matrix effects – we recommend using LowCross-Buffer®.
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文献和实验3.0 ml ethanolamine before use(10) 1X Buffer A0.1 M NaOAc 0.82 g NaOAc0.5 M NaCl 2.92 g NaClpH to 4.0 and bring up to 100 ml2. Procedure(1) Pour a 5 ml affigel blue column (biorad) and wash with 50 ml Prewash to prep the column (when first used
Polyclonal Antibody Production
For injection of two rabbits, your antigen should be in 1 mL of a buffer such as PBS or Tris that contains no chemicals harmful to the rabbit. 100 µg/rabbit of antigen is best, but you can use less and get good results.
IMMUNOSCREENING OF WITH ANTIBODY PROBES
Adsroption of Anti-E. coli Antibodies Grow E. coli strain Y1090 in L broth at 37°C overnight. Transfer to 10 mM MgSO4 and store at 4°C for future use. These cells are good for about 1 week. Soak one 132 mm nitrocellulose membrane per Petri dish










