产品封面图

DNA Ladder Detection Kit; 50 a

ssays
收藏
  • 询价
  • APT151
  • 2026年01月11日
  • Activity Assay
  • 0
  • Mammals
avatar
11金牌会员
  • 企业认证

    点击 QQ 联系

    • 详细信息
    • 询价记录
    • 文献和实验
    • 技术资料
    • 应用范围

      Activity Assay

    • 宿主

      0

    • 抗原来源

      0

    • 库存

      大量

    • 适应物种

      Mammals

    • 是否单克隆

      0

    • 规格

      50 assays

    Description:
    DNA Ladder Detection Kit
    Trade Name:
    Chemicon (Millipore)
    Product Overview:
    Internucleosomal DNA fragmentation is a hallmark of apoptosis in mammalian cells. Chemicon's Quick Apoptotic DNA Ladder Detection Kit provides a fast and easy means for detection of DNA fragmentation in apoptotic cells. Unlike other similar kits which require 1-2 days to finish the procedure, the new detection method requires less than 90 minutes to prepare DNA in a single tube, without the need for extraction or the use of columns. DNA fragmentation can be easily visualized by agarose gel electrophoreses. The new procedure increases recovery of small fragmented DNA and therefore improves the sensitivity of the assay.
    Key Applications:
    Activity Assay
    Species Reactivity:
    Mammals
    Usage Statement:
    Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    Kit or Assay Type:
    • Apoptosis Assays
    • Apoptosis Assays
    Components:
    • TE Lysis Buffer - 2 mL.
    • Enzyme A Solution - 0.25 mL.
    • Enzyme B* - 1 vial
    • Ammonium Acetate Solution - 0.25 mL.
    • DNA Suspension Buffer - 2 mL.
    • Note: Dissolve Enzyme B with 275 μL TE Lysis Buffer and mix well before use. The unused Enzyme B solution should be frozen at -70°C.
    Storage Conditions:
    We recommend storing reagents at -20°C upon arrival. Frequent freeze-thawing is not recommended. The performance of this product is guaranteed for 6 months.
    Detection Methods:
    Chromogenic
    Materials Required but Not Delivered:
    · Microcentrifuge and 1.5 mL microcentrifuge tubes

    · 55°C water bath or incubator

    · Phosphate buffered Saline solution (PBS) - [10 mM Na2PO4; 138 mM NaCl; 2.7 mM KCl]

    · Absolute ethanol

    · Equipment and reagents for agarose gel electrophoresis and DNA visualization

    更多产品技术资讯,请访问密理博中国博客:http://blog.milliporechina.com

    详细描述见链接:http://www.millipore.com/catalogue/item/APT151

    风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。

    • 作者
    • 内容
    • 询问日期
    图标文献和实验
    相关实验
    • DNA LADDER检测细胞凋亡的讨论

      -2等!这也是比较常见的! fffwu:我做的是内皮细胞凋亡,ecv-304,我用的是试剂盒,申能博彩的。dna的提取没问题, 但是我跑不出ladder,我用的是40v,1小时,胶的浓度是1。5%, midas:增加时间到3h,胶的浓度可以减少到0.8-1.0% lxh_lily:做过几次DNA ladder了,光镜下看到50%的细胞都脱落了,但是电泳结果只能看到一大片很亮的拖带,不能分出一条一条的ladder,我用的是1.2%的琼脂糖凝胶,恒流,60mA。是不是用page电泳效果会好一点? midas

    • Detection of apoptotic process in situ using immunocytochemical and TUNEL assays

      in individual apoptotic cells by the in situ terminal deoxynucleotidyl transferase and nick translation assays. Cancer Res . 52: 1945. 3. Gorczyca, W.,Tuziak,T., Kram, A., Melamed, M.R., Darzynkiewicz, Z. 1994. Detection of apoptosis-associated DNA strand

    • 制作DNA ladder常见问题

      的是内皮细胞凋亡,ecv-304,我用的是试剂盒,申能博彩的。dna的提取没问题, 但是我跑不出ladder,我用的是40v,1小时,胶的浓度是1。5%, midas:增加时间到3h,胶的浓度可以减少到0.8-1.0% lxh_lily:做过几次DNA ladder了,光镜下看到50%的细胞都脱落了,但是电泳结果只能看到一大片很亮的拖带,不能分出一条一条的ladder,我用的是1.2%的琼脂糖凝胶,恒流,60mA。是不是用page电泳效果会好一点? midas:看来

    图标技术资料

    暂无技术资料 索取技术资料

    同类产品报价

    产品名称
    产品价格
    公司名称
    报价日期
    ¥4884
    北京智杰方远科技有限公司
    2025年07月14日询价
    $320
    上海瑶韵生物科技有限公司
    2025年06月10日询价
    询价
    默克生命科学
    2026年01月11日询价
    询价
    美国CST中国公司(丁香通商铺)
    2025年09月22日询价
    询价
    上海北诺生物科技有限公司
    2025年07月14日询价
    DNA Ladder Detection Kit; 50 assays
    询价