- 询价
- AB5895
- 2026年01月04日
- Western Blotting;Immunocytochemistry
- Rabbit
- Mouse;Rat
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- 详细信息
- 文献和实验
- 技术资料
- 应用范围：
Western Blotting;Immunocytochemistry
- 宿主：
Rabbit
- 抗原来源：
0
- 库存：
大量
- 适应物种：
Mouse;Rat
- 是否单克隆：
2
- 规格：
50 μL
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, R | IC, IH, WB | Rabbit | Serum | Polyclonal Antibody |
SPECIES REACTIVITIES:
Rat, mouse and primate. Other species have not been tested.
- Immunocytochemistry
- Immunohistochemistry
- Western Blotting
Immunohistochemistry: 1:500-1:1,000
Immunocytochemistry: 1:500-1:1,000
Optimal working dilutions must be determined by the end user.
APPLICATION NOTES FOR AB5895
IMMUNOHISTOCHEMISTRY
Male Sprague-Dawley rats (b.wt. 100-150g) were anesthetized with sodium pentobarbital and perfused via the ascending aorta with: 1) 50 mL of Ca2+-free Tyrode+s solution followed by 2) fixative, and 3) 10% sucrose in PBS as a cryo-protectant. Tissues were rapidly dissected out and stored overnight in 0.1 M phosphate buffer (pH 7.4) containing 10% sucrose.
Slide-mounted tissue sections were incubated with blocking buffer for 1 hour at room temperature. Primary antibody was diluted in blocking buffer to the appropriate working dilution. Blocking buffer was removed and the slides were then incubated at 2-8°C for 18-24 hours with AB5895. After rinsing in PBS 3 times sections were incubated for 60 minutes at room temperature with Cy3-conjugated secondary antibodies. After mounting in a mixture of PBS and glycerol (1:3) containing 0.1% p-phenylenediamine, sections were examined with a Nikon Microphot-SA epifluorescence microscope.
IMMUNOCYTOCHEMISTRY
P2X3 transfected cells were processed for indirect immunofluorescence. Media was removed and cells were gently washed 3 times with serum-free media. Following fixation procedure, cells were processed for indirect immunofluorescence as described above.
- Mouse
- Rat
- P2RX3
- MGC129956
- P2X3
- Neurotransmitters & Receptors
- Neuroinflammation & Pain
更多产品技术资讯，请访问密理博中国博客：http://blog.milliporechina.com
Synthetic peptide corresponding to the C-terminus of rat P2X3. 详细描述见链接：http://www.millipore.com/catalogue/item/AB5895
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