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Caspase 3 Colorimetric Activit

y Assay Kit, DEVD; 25 assays
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  • 询价
  • APT131
  • 2025年07月04日
  • Activity Assay
  • 0
  • Mammals
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 应用范围：

      Activity Assay

    • 宿主：

      0

    • 适应物种：

      Mammals

    • 库存：

      大量

    • 抗原来源：

      0

    • 是否单克隆：

      0

    • 规格：

      25 assays

    Description:
    Caspase 3 Colorimetric Activity Assay Kit, DEVD
    Trade Name:
    Chemicon (Millipore)
    Product Overview:
    Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. Chemicon's Caspase-3 Colorimetric Activity Assay Kits provide a simple and convenient means for assaying the activity of caspases that recognize the sequence DEVD. The assay is based on spectophotometric detection of the chromophore p-nitroaniline (pNA) after cleavage from the labeled substrate DEVD-pNA. The free pNA can be quantified using a spectrophotometer or a microtiter plate reader at 405 nm. Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase-3 activity.
    Key Applications:
    Activity Assay
    Species Reactivity:
    Mammals
    Usage Statement:
    Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    Kit or Assay Type:
    • Apoptosis Assays
    • Caspase Assays
    • Apoptosis Assays
    Components:
    • 5X Cell Lysis Buffer (Part No. 90065): 5 mL
    • 5X Assay Buffer (Part No. 90066): 10 mL
    • Caspase-3 Substrate (Ac-DEVD-pNA) (Part No. 90079): 250 μL of 3 mg/mL solution
    • Caspase-3 Inhibitor (Ac-DEVD-CHO) (Part No. 90067): 50 μL of 100 μM (0.05 mg/mL) in DMSO
    • pNA Standard (Part No. 90085): 250 μL of 10 mM in DMSO
    Storage Conditions:
    Store kit materials at -20°C up to their expiration date.

    Special Precautions:

    · After thawing reagents, use immediately or aliquot and freeze at -20°C for longer storage. Avoid repeated freeze/thaw cycles.

    · The Caspase-3 substrate and pNA standard are especially light sensitive. Maintain these reagents in amber or covered containers.
    Detection Methods:
    Chromogenic
    Gene Symbol:
    • CASP3
    • apopain
    • procaspase3
    • CPP-32
    • Apopain
    • Yama
    • CASP-3
    • CPP32B
    • SCA-1
    • CPP32
    • EC 3.4.22.56 [Contains: Caspase-3 subunit p17
    • Caspase-3 subunit p12].
    Alternate Names:
    CPP32
    Materials Required but Not Delivered:
    · Microcentrifuge and 1.5 mL Microcentrifuge tubes

    · 37°C Waterbath or Incubator

    · Spectrophotometer or Microplate Reader

    · 96 well Microtiter Plate

    更多产品技术资讯，请访问密理博中国博客：http://blog.milliporechina.com

    详细描述见链接：http://www.millipore.com/catalogue/item/APT131

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