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Goat anti-Mouse IgG, (H+L) HRP

conjugate; 1 mL
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  • 询价
  • AP308P
  • 2025年07月11日
  • ELISA;Western Blotting
  • Goat
  • Mouse
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 应用范围

      ELISA;Western Blotting

    • 宿主

      Goat

    • 适应物种

      Mouse

    • 库存

      大量

    • 抗原来源

      0

    • 是否单克隆

      2

    • 规格

      1 mL

    Species Reactivity Key Applications Host Format Antibody Type
    M ELISA, IC, IH, WB Goat HRP Polyclonal Antibody
    Description:
    Goat anti-Mouse IgG, (H+L) HRP conjugate
    Background Information:
    The reagent is an affinity purified antibody from goat. The purified antibody is conjugated to horseradish peroxidase (HRP) and stabilized in buffer.
    Specificity:
    Specific for mouse IgG, heavy and light chain. The cross-reactivities of anti-mouse IgG antibody are tested in an ELISA. Minimum cross-reactivity to human IgG.
    Key Applications:
    • ELISA
    • Immunocytochemistry
    • Immunohistochemistry
    • Western Blotting
    Application Notes:
    ELISA:
    1:3,000 dilution can be used (Coligan, J., 1997; Harlow, E., 1988).

    Immunohistochemistry/immunocytochemistry:
    1:200-1:500 dilution can be used (Bullock, G.R., 1982; Javois, L.C., 1994).

    Optimal working dilutions must be determined by the end user.
    Species Reactivity:
    Mouse
    Isotype:
    IgG
    Presentation:
    Purified Goat IgG in buffer containing liquid in PBS, pH 7.1 with 15 mg/mL BSA and 0.01% thimerosal.
    Storage Conditions:
    The undiluted antibody is stable at 2-8°C for 12 months. Do not store in a diluted format. DO NOT FREEZE.
    Quality Assurance:
    Western Blot, chromogenic:
    1:200-1:500 dilution can be used (Coligan, J., 1997; Harlow, E., 1988).

    Western Blot, chemiluminescent:
    1:2,000-1:5,000 dilution can be used (Coligan, J., 1997).
    Antibody Category:
    Secondary & Control Antibodies
    Antibody Sub-Category:
    Whole Immunoglobulin Secondary Antibodies
    Usage Statement:
    Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    Trade Name:
    Chemicon (Millipore)
    Format:
    HRP
    Host:
    Goat
    Product Overview:
    The reagent is an affinity purified antibody from goat. The purified antibody is conjugated to horseradish peroxidase (HRP) and stabilized in buffer.
    Concentration:
    1 mg/mL
    Antibody Type:
    Polyclonal Antibody

    更多产品技术资讯,请访问密理博中国博客:http://blog.milliporechina.com

    详细描述见链接:http://www.millipore.com/catalogue/item/AP308P

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    图标文献和实验
    相关实验
    • 人抗风疹病毒IgG抗体(anti-RV IgG)酶联免疫分析(ELISA)

      中依次加入抗风疹病毒 IgG 抗体( anti-RV IgG ),再与 HRP 标记的抗原结合,形成抗原 - 抗体 - 酶标抗原复合物,经过彻底洗涤后加底物 TMB 显色。 TMB 在 HRP 酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的抗风疹病毒 IgG 抗体( anti-RV IgG )呈正相关。用酶标仪在 450nm 波长下测定吸光度( OD 值),通过标准曲线计算样品中人抗风疹病毒 IgG 抗体( anti-RV IgG )浓度。 试剂盒组成

    • E L I S A w i t h p l a t e l e t s

      for 1 hour with a 1 :1000 dilution of rat IgG2 a anti-mouse kappa chain monoclonal antibody conjugated to horse-radish peroxidase (LO-MK1 , LO/IMEX) reveal with o-phenylenediamine dihydrochloride (OPD) in revelation solution. SOLUTIONS

    • AbC Anti-Mouse Bead Kit

      the bead pellet by adding 0.5 mL of buffer to each sample tube. 11)    Add one drop of negative beads (Component B in the AbC™ Anti-Mouse Bead Kit) to sample tube(s) containing the AbC™ capture beads. 12)    Add one drop of ArC™ negative beads

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