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- 技术资料
- 保存条件:
2-8°C
- 保质期:
根据瓶身LOT号查询
- 英文名:
1-Thioglycerol
- 库存:
有现货
- 供应商:
浙江羽翔生物科技有限公司
- CAS号:
9002-93-1
- 规格:
25ML
属性
生物来源
synthetic (organic)
质量水平
200
产品线
BioReagent
方案
≥97% (titration)
表单
liquid
技术
cell culture | mammalian: suitable
single cell analysis: suitable
折射率
n20/D 1.527 (lit.)
沸点
118 °C/5 mmHg (lit.)
溶解性
water: miscible 0.1 M
密度
1.25 g/mL at 25 °C (lit.)
储存温度
2-8°C
SMILES字符串
OCC(O)CS
InChI
1S/C3H8O2S/c4-1-3(5)2-6/h3-6H,1-2H2
InChI key
PJUIMOJAAPLTRJ-UHFFFAOYSA-N
应用
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文献和实验Single-cell transcriptomics identifies CD44 as a marker and regulator of endothelial to haematopoietic transition.
The endothelial to haematopoietic transition (EHT) is the process whereby haemogenic endothelium differentiates into haematopoietic stem and progenitor cells (HSPCs). The intermediary steps of this process are unclear, in particular the identity of endothelial cells that give rise to HSPCs is unknown. Using single-cell transcriptome analysis and antibody screening, we identify CD44 as a marker of EHT enabling us to isolate robustly the different stages of EHT in the aorta-gonad-mesonephros (AGM) region. This allows us to provide a detailed phenotypical and transcriptional profile of CD44-positive arterial endothelial cells from which HSPCs emerge. They are characterized with high expression of genes related to Notch signalling, TGFbeta/BMP antagonists, a downregulation of genes related to glycolysis and the TCA cycle, and a lower rate of cell cycle. Moreover, we demonstrate that by inhibiting the interaction between CD44 and its ligand hyaluronan, we can block EHT, identifying an additional regulator of HSPC development.
licanming 请问大家用sigma公司的PMA时,储存液的浓度是配多少的?保存期多长?我是准备用DMSO配,我看说明书的意思是储存液浓度为20mM,但我工作液的浓度只要100nM,故很难分装和稀释。如果储存液浓度低一点,会不会对保存时间产生影响啊?我在园子里搜过,有用1mg/ml的,但我不知他是否用sigma公司的。 licanming 自己顶一下 amygdala 对于保存
:Formerly listed as Type III 溶解性:溶于水,参考浓度2mg/ml 储存条件:2-8℃ From Sigma D1626 Salmon DNA 的配制: 组份浓度10 mg/ml Salmon DNA ;配制量约100 ml 配制方法: 1. 称取鲑鱼精DNA 2 g置于500 ml烧杯中,加入约200 ml的TE Buffer。 2. 用磁力搅拌器室温搅拌2~4小时
、在立体显微镜下,可以看到明显的 3D 结构。使用 200μl 移液器吸头从每个孔中收集球状体,并将大约 50 个球状体集中到 5ml 微量离心管中。 中肠和后肠球状体生长成人肠类器官 11、收集球状体后,将微量离心管垂直放置在管架上 10min,此时球体通过重力沉降到离心管底部。吸出上清,控制总体积在 25μl 左右。 12、将冻存的 Matrigel 在 4℃ 下过夜解冻,添加 B-27 补充剂(终浓度 1×)、R-spondin1 (终浓度 500ng/ml),Noggin (终浓度100
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