- ￥1791
- Sigma-Aldrich
- 进口
- M9508-5G
- 2025年07月13日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
−20°C
- 保质期：
根据瓶身LOT号查询
- 英文名：
Methylenediphosphonic acid
- 库存：
有现货
- 供应商：
浙江羽翔生物科技有限公司
- CAS号：
1984-15-2
- 规格：
5G
属性
质量水平
100
方案
≥99%
表单
crystals
mp
197-199 °C (lit.)
储存温度
−20°C
SMILES字符串
OP(O)(=O)CP(O)(O)=O
InChI
1S/CH6O6P2/c2-8(3,4)1-9(5,6)7/h1H2,(H2,2,3,4)(H2,5,6,7)
InChI key
MBKDYNNUVRNNRF-UHFFFAOYSA-N
应用
- 在烷基三甲基铵表面活性剂存在的情况下制备介孔有机膦suan铝
- MDP酯的四酯，其在所有氧化态下均可用作锕系元素的金属离子萃取剂
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文献和实验Globular structures in roots accumulate phosphorus to extremely high concentrations following phosphorus addition.
Crops with improved uptake of fertilizer phosphorus (P) would reduce P losses and confer environmental benefits. We examined how P-sufficient 6-week-old soil-grown Trifolium subterraneum plants, and 2-week-old seedlings in solution culture, accumulated P in roots after inorganic P (Pi) addition. In contrast to our expectation that vacuoles would accumulate excess P, after 7 days, X-ray microanalysis showed that vacuolar [P] remained low (<12 mmol kg-1 ). However, in the plants after P addition, some cortex cells contained globular structures extraordinarily rich in P (often >3,000 mmol kg-1 ), potassium, magnesium, and sodium. Similar structures were evident in seedlings, both before and after P addition, with their [P] increasing threefold after P addition. Nuclear magnetic resonance (NMR) spectroscopy showed seedling roots accumulated Pi following P addition, and transmission electron microscopy (TEM) revealed large plastids. For seedlings, we demonstrated that roots differentially expressed genes after P addition using RNAseq mapped to the T. subterraneum reference genome assembly and transcriptome profiles. Among the most up-regulated genes after 4 hr was TSub_g9430.t1, which is similar to plastid envelope Pi transporters (PHT4;1, PHT4;4): expression of vacuolar Pi-transporter homologs did not change. We suggest that subcellular P accumulation in globular structures, which may include plastids, aids cytosolic Pi homeostasis under high-P availability.
.14 Just to check enzyme and color product put 100 µl of each in a test tube and watch for a yellow color change.Figure 1. Schematic of an ELISAMATERIALSPhosphate-buffered saline (PBS) ,0.01 M Na2HPO4,0.15 M MaCl, PH 7.4Sodium phosphate dibasic (Na2HPO
DIRECT AND SHORT-TERM PROCEDURE FOR HARVESTING BONE MARROW CHROMOSOMES
prepared fresh. Hypotonic solution 0.075 M KCl: 2.8 g Potassium chloride dissolved in 500 ml dH2O. VII. Reagents: Colcemid: GIBCO cat# 890-3014. 10 mg bottle. Ethidium Bromide: SIGMA cat# E-8751. 2,7-diamino-10-ethyl-9-phenyl
TISSUE FIBROBLAST CULTURES FOR CHROMOSOME ANALYSIS
2 ml of fixative and mix. Let sit at room temperature 15 - 20 minutes. M. Repeat steps K,L 1 or 2 times until the pellet is clean and white. The culture is now ready to make slides. For best results, slides should be made the same day
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