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- 详细信息
- 技术资料
- 宿主:
Rabbit
- 抗原来源:
0
- 适应物种:
Human
- 库存:
大量
- 是否单克隆:
2
- 规格:
500 μL
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | ELISA, IF, not WB, not IH(P) | Rabbit | Purified | Polyclonal Antibody |
Description:
Anti-Collagen Type III
Specificity:
The antibody reacts with native and heat denatured (non-reduced) human collagen type III. Cross reactions with other types of collagen under native conditions do occur. There is 10% cross reactivity with human collagen type I, 2% cross reactivity with human collagen type II, and 4% cross reactivity with human collagen type IV and V. There is no cross reactivity with other human plasma proteins under native ELISA conditions. Reactivity under denatured conditions has not been examined.
Cross Reactivity Percent
Human collagen type I 10%
Human collagen type II 2%
Human collagen type III 100%
Human collagen type IV and V 4%
Human plasma proteins do not interfere with binding to collagen.
Cross Reactivity Percent
Human collagen type I 10%
Human collagen type II 2%
Human collagen type III 100%
Human collagen type IV and V 4%
Human plasma proteins do not interfere with binding to collagen.
Key Applications:
- ELISA
- Immunofluorescence
Applications Not Recommended:
- Western Blotting
- Immunohistochemistry (Paraffin)
Application Notes:
ELISA 1:3,000
Indirect immunofluorescence on cryostat sections or cultured cells 1:20-1:40
Optimal working dilutions must be determined by the end user.
Indirect immunofluorescence on cryostat sections or cultured cells 1:20-1:40
Optimal working dilutions must be determined by the end user.
Species Reactivity:
Human
Immunogen:
Human placental collagen type III
Presentation:
500ul containing of 0.15M NaCl, 10 mM sodium phosphate pH 7.5, with 0.1% mannitol and o.1% dextran as stabilizers contains no preservatives.
Immunoglobulin fraction was prepared by ammonium sulfate precipitation and chromatography on DEAE-cellulose, of antiserum cross-absorbed over immobilized human serum proteins, immunoglobulins, and collagen types I, II, IV and V.
Immunoglobulin fraction was prepared by ammonium sulfate precipitation and chromatography on DEAE-cellulose, of antiserum cross-absorbed over immobilized human serum proteins, immunoglobulins, and collagen types I, II, IV and V.
Storage Conditions:
Antibody is stable for 12 months from date of receipt when stored at 2-8ºC. Remove any nondispersed aggregates by microcentrifugation prior to use.
Gene Symbol:
- COL3A1
- EDS4A
- FLJ34534
Antibody Category:
Cell Structure
Antibody Sub-Category:
ECM Proteins
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Trade Name:
Chemicon (Millipore)
Format:
Purified
Host:
Rabbit
Antibody Type:
Polyclonal Antibody
Product Family Information
Collagen AntibodiesMillipore’s Collagen antibody demonstrates specificity against collagen. See below for data, references and related products for collagen. All Millipore antibodies are based on the expertise of Upstate & Chemicon. |
更多产品技术资讯,请访问密理博中国博客:http://blog.milliporechina.com
Human placental collagen type III 详细描述见链接:http://www.millipore.com/catalogue/item/AB747
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Anti-Collagen Type III; 500 μL
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