- 询价
- AB5804
- 2026年01月03日
- Immunocytochemistry;Immunohistochemistry
- Rabbit
- Bovine;Canine;Human;Rat
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- 详细信息
- 文献和实验
- 技术资料
- 应用范围：
Immunocytochemistry;Immunohistochemistry
- 宿主：
Rabbit
- 抗原来源：
0
- 库存：
大量
- 适应物种：
Bovine;Canine;Human;Rat
- 是否单克隆：
2
- 规格：
50 μL
Rabbit anti-GFAP (Catalog Number AB5804) and Chicken anti-NF-H (Catalog Number AB5539). Staining of cultured neonatal rat forebrain with the anti-GFAP (green) and anti-NF-H (red). The blue is Hoechst DNA stain.
Chicken anti-Neurofilament H (Catalog Number AB5539) localization of NF-H (green) in cultured neonatal rat forebrain cells. Red staining in GFAP (Catalog Number AB5804). The blue is Hoechst DNA stain.
Chicken anti-Vimentin (Catalog Number AB5733). Localization of Vimentin (green) and GFAP (red, AB5408) in rat cerebral cortex neurons. Note flattened fibroblastic cells are mostly green (i.e. vimentin positive, GFAP negative), while clearly astrocytic cells, express both vimentin and GFAP and therefore appear golden or orange. Certain other cells express predominantly GFAP and therefore appear red. Photo courtesy of EnCor Biotechnology, Inc.
Rabbit anti-GFAP (Catalog Number AB5804). Staining of cultured neonatal rat forebrain with anti-GFAP is shown in green, while neuronal processes were identified using a monoclonal antibody to the neurofilament subunit alpha-internexin (Catalog Number MAB5224) and are shown in red.
Rabbit anti-GFAP (Cat. No. AB5804). Primary rat hippocampus-derived embryonic astrocytes (Catalog Number SCR008) stained with rabbit anti GFAP (red) and mouse anti MAP2 (green, MAB3418-50UG). Neurons were not observed as evidenced by the lack of MAP2 staining. Cell nuclei were counterstained with DAPI (blue).
Immunohistochemistry(paraffin): Representative images from a previous lot.
Pictures of Optimal Staining With Citrate Buffer Epitope Retrieval: Human Brain
GFAP (IHC2078) staining of Human Brain. Tissue pretreated with Citrate Buffer, pH 6.0. Pre-diluted polyclonal antibody, using IHC-Select® Detection with HRP-DAB. Glial cells stain strongly (brown). Bar = 25 μm.
Immunohistochemistry(paraffin): Representative images from a previous lot.
Pictures of Optimal Staining With Citrate Buffer Epitope Retrieval: Human Brain
Negative Control staining of Human Brain. Tissue pretreated with Citrate Buffer, pH 6.0. Negative Control Reagent, IHC-Select® Detection with HRP-DAB. No background staining is detected. Bar = 25 μm.
Immunohistochemistry(paraffin): Representative images from a previous lot.
Pictures of Optimal Staining With Citrate Buffer Epitope Retrieval: Human Brain
GFAP (IHC2078) staining of Human Brain. Tissue pretreated with TE Buffer, pH 9.0. Pre-diluted polyclonal antibody, IHC-Select® Detection with HRP-DAB. Glial cells stain very strongly (brown). Bar = 100 μm.
Immunohistochemistry(paraffin): Representative images from a previous lot.
Pictures of Optimal Staining With Citrate Buffer Epitope Retrieval: Human Brain
Negative Control staining of Human Brain. Tissue pretreated with TE Buffer, pH 9.0. Negative Control Reagent, IHC-Select® Detection with HRP-DAB. No background staining is detected. Bar = 100 μm.
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| B, Ca, H, R | IC, IH, IH(P) | Rabbit | Serum | Polyclonal Antibody |
- Immunocytochemistry
- Immunohistochemistry
- Immunohistochemistry (Paraffin)
Representative images from a previous lot.
Immunocytochemistry:
A 1:1,000 dilution of a previous lot was used in Immunocytochemistry.
- Bovine
- Canine
- Human
- Rat
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
- FLJ45472
- GFAP
Immunohistochemistry(paraffin) Analysis:
GFAP (IHC2078) staining of Human Brain. Tissue pretreated with Citrate Buffer, pH 6.0. Pre-diluted polyclonal antibody, using IHC-Select® Detection with HRP-DAB. Glial cells stain strongly (brown).
Optimal Staining with Citrate Buffer Epitope Retrieval: Human Brain
Product Family Information
GFAP Antibodies & KitsMillipore’s Anti-GFAP antibody demonstrates specificity against GFAP. See below for data, references and related products for GFAP. All Millipore antibodies are based on the expertise of Upstate & Chemicon. |
更多产品技术资讯，请访问密理博中国博客：http://blog.milliporechina.com
Purified bovine GFAP. 详细描述见链接：http://www.millipore.com/catalogue/item/AB5804
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